Tanzan C8- i3 Column

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• Related Substances Of Furosemide (Frusemide)
• Application Of Uracil and Alkylbenzenes

General HPLC Column Care And Use Instructions
Column Storage and Column Care
General Guidelines
Do not store HPLC columns in buffers. A buffer may precipitate inside the column, resulting in plugged hits and packing material. A buffer may also encourage bacterial growth, which can plug both the column frit and packing material. This is more likely to occur with mobile phases which have close to or 100% buffer. Bacteria may also affect your analytes, and organic products from the dead bacteria may cause “ghost peaks” in chromatograms. Do not store HPLC columns in solvents that degrade easily — tetrahydrofuran (THF), triethylamine (TEA), and trifluoroacetic acid (TFA). Unstabilized THF can form peroxides which may degrade the column. TEA and TFA are more likely to become contaminated from the lab environment and should be refrigerated during storage. Contamination may change the chromatography or change the column. Be aware of the quality issues with these mobile phase components when you use them for your chromatography.

Term Storage

Reversed-phase columns can also be stored in 100% acetonitrile. If you are using a buffered mobile phase, first flush the column with the mobile phase minus the buffer, then with 100% acetonitrile. In addition, a 100% ACN flush prior to long term storage can help remove contaminants from the column.

Regeneration of RP packings

RP- packings are C18, C8, C4, C1, C30, CN or Phenyl stationary phases
• Flush the column with 20? column volumes Water
• Flush the column with 20? column volumes Acetonitrile
• Flush the column with 5? column volumes Isopropanol
• Flush the column with 20? column volumes Heptane
• Flush the column with 5? column volumes Isopropanol
• Flush the column with 20 column volumes Acetonitrile

Regeneration of Ion-Exchange packings

Anion- and Cation-Exchangers (WCX, SCX, WAX and SAX) are Ion-Exchange Packings
• Wash with 20 column volumes with the eluent, double the buffer concentration
• Wash with 20 column volumes Water (to elute out salts)
• Wash with 20 column volumes Acetonitrile (to elute hydrophilic contaminants)
• Wash with 5 column vol. Isopropanol (to elute mildly hydrophobic contaminants)
• Wash with 20 column volumes Heptane or Hexane or Methylenchloride (to elute strongly hydrophobic contaminants)
• Wash with 5 column volumes Isopropanol
• Wash with 20 column volumes Acetonitrile
• Wash with 20 column volumes Water
• Return the column to its original Ion-Exchange condition